1U6 



THE BOTAJ^WAL MAGAZINE. 



[Vol. XXXIII. No. an. 



17 



18 

 19 

 20 

 21 

 22 

 23 

 24 

 25 

 26 

 27 

 28 

 29 

 30 







6 



2 



1 











2A 



18 + 2A 

 12 











24-3A 



3 







+ + + ( 



Total 



57+19.\ 



It might be doubted that the injur}' made by plasm olysis would be 

 the cause of growth ("Wundreiz"). The removal of growmg-pomt, for 

 example causes adventitious growth in the fern prothallia (Goebel : 

 1902), but such is, of course, not the case here. There was a tendency, 

 however, that the partially injured gemmae formed more adventitious 

 thalli from the living portion than the normally recovered ones, but 

 mere mechanical injury did not induce adventitious growth. The 

 gemmae were punctured by the sharp needle and let to grow on the 

 nutrient solution. None of them formed adventitious thalli. The 

 removal of water from the cell would not be the cause either. Merc 

 drying the gemmae by exposing them to strong day light on the moist 

 filter paper showed no change either in the direction or manner of 

 growth. It seems more likely that the stimuli might be due to the 

 change of "structure" of protoplasm (Wakburg). The sequence of 

 events in the treated cell of gemma may be compared with those of the 

 sea urchin egg which is treated by the liypertonic sea water. 



It is important for us to know the effect of plasmolysis on the plant 

 cell. Studies made by Gaidukov (1910), Hecht (1912) and Price 

 (1913) showed that the plasma-membrane of plant cell which is com- 

 posed of hydro-sol was changed to gel as soon as the hj'pertonic 

 solution was applied to the cell. When the protoplasm was pulled apart 

 from the cell wall, the pieces were retained on the wall and a number of 

 fine protoplasmic fibriles were formed between the wall and the con- 

 tracted mass. They were in rapid movement and were considered to 



10 One apex injured. 11 Prothallia-like growth. 



