On the Localisation of Anthocyanin in the 



Spring Leaves of Some Trees and Shrubs 



in the Temperate Regions of Japan. 



by 

 Tsiitsuiui lehiiniira, 



{Professor of Botany in F'ourth Higher School, A'a/iasatta, Japan.) 



The reddening of young leaves just developing in the spring time 

 is so remarkable in the vicinity of Kanazawa that ever\'man never 

 fails to observe it. Microscopical examinations of the tissues containing 

 red pigment (anthocyanin) evince that they hold the pigment in solu- 

 tion in their cell-sap, which, of course, occupies the vacuole of the. 

 cells. My observations on the histological distribution of such periodic 

 anthoc3-anin have been made on the trees and shrubs in that locality, 

 which turn red most conspicuously. 



The localisation of the pigment in the leaves is as follows, the 

 number of genera being 44 in all : — 



