333] STUDIES ON MYXOSPORIDIA—KUDO 95 



rounded by several layers (about lO^c thick) of connective tissue of the 

 host, the other oval, 120m by llO/x. The staining sufficed to reveal only 

 indistinct structure of the parasites. The homogeneous ectoplasm sur- 

 rounds the entire surface of the body as a uniform, but very thin layer. 

 Endoplasm granular, filled with spores of remarkably identical stages of 

 development. Isolated spores, also, occur in the muscle bundle in the 

 state of diffused infiltration. Polysporous. 



Spore: Hahn describes it as follows: Low conical pyramid with round 

 base; square with bulging sides. No indication of valves in the spore 

 shell. Dimensions: height (length) 5fx, breadth and thickness 7/i, polar 

 capsule 2/i by In. 



Linton's form: squarish in outline with rounded corners, 7^ in di- 

 ameter. 



Tyzzer describes his form as follows: Quadrilateral in anterior end 

 view; oval in side view. The four corners are a little protuberant and are 

 directed slightly forward. Shape varies considerably in different species 

 of host. The corners of the spore from Stenotomus chrysops, are greatly 

 drawn out, exhibiting stellate form. Four polar capsules radiating from 

 the anterior extremity toward the four corners. Shell shows four furrows 

 radiating from the anterior extremity outwards to the side. Sporoplasm 

 occupies extracapsular cavity. Polar filaments are extruded under the 

 action of acetic acid. Dimension: breadth 7 to 7.5/i. 



The writer's observations are as follows: 



Spores in fixed and decolorized smears. In smear, most of the spores 

 are seen lying on the base exposing the anterior end view toward the 

 observer's eyes, a few lying with the sutural diameter parallel to the surface 

 of the slide. Form quadrilateral with corners more or less drawn out in 

 anterior end view; oval, with concave posterior side in front view (Figs. 

 562 to 564). Shell apparently thin but was not clearly separated from the 

 sporoplasm which is finely granular and fills the extracapsular cavity of 

 the spore. Four polar capsules of nearly same size and pyriform. Coiled 

 polar filament indistinct. When stained, the polar capsules stained deeply. 

 It is remarkable to see almost all of the spores exhibit four deeply stained 

 nuclei of capsulogenous cells, which in ordinary case disappear as the spore 

 matures. Dimensions: height (length) 4 to 4.75^, breadth and thickness 

 5.4 to 6.5/x, polar capsule about 1.5/u by 0.75^. 



Remarks: Thus the forms of Tyzzer, Linton and Hahn had better 

 be treated as one and the same species. As to the distinction of Chloro- 

 myxum funduli and the present species, the writer is unable to make it 

 clear as he could not examine the preparation of the former species and 

 especially as he observed some intermediate forms between these two 

 forms in Dr. Tyzzer's preparations of the present species. 



