411] STUDIES ON MYXOSPORIDIA— KUDO 173 



HENNEGUYA MICTOSPORA nov. spec. 

 [Figs. 546 to 557] 



Habitat: Urinary bladder of Lepomis cyanellus Raf., L. humilis Gir. 

 and Micropterus sahnoides Lac; Stony Creek, 111. (November). 



In one out of three (6.5 to 8cm. long), of the first, in one out of two 

 (7 and 9.5cm. long) of the second and in one of the third species, examined 

 in the middle of November, was found the present form. None showed a 

 heavy infection, a number of scattered trophozoites and spores being, 

 observed. The host did not show any pathological change. 



Vegetative form : Polymorphous. Generally rounded or elongated oval. 

 In small monosporous and disporous forms, the tail of the spores developed 

 inside, is extruded from the body, so that these trophozoites show long 

 processes (Figs. 546, 553, 555). Pseudopodia lobose, and extruded from the 

 entire surface of the body (Fig. 547), tho sometimes they are well formed 

 at one end of the body. Protoplasm is differentiated distinctly into ecto- 

 plasm and endoplasm. Ectoplasm is homogeneous and hyaline, forming 

 the outer layer. Endoplasm is of reticular structure. The body is colorless, 

 often yellowish, when the endoplasm is loaded with numerous yellowish 

 coarse granules. The size varies from 6 or 7/x up to 60/i. In a rounded form 

 of 38/i in longest diameter, five pansporoblasts, each developing two spores 

 and many nuclei were observed. In another oval form of 45/t by 60/i in 

 size, numerous nuclei were stained, showing that no development of 

 pansporoblast has yet taken place. Disporous, polysporous and mono- 

 sporous, tho of rare occurrence. 



Spore: Broad spindle shape with attenuated anterior end. Shell rather 

 thin. Each valve has 6 to 8 longitudinal striations on the surface. A long 

 tail composed of two halves, is developed at the posterior end. Two pyri- 

 form polar capsules with distinctly visible coiled polar filament opens at 

 the anterior tip. Sporoplasm, finely granular, contains an iodinophilous 

 vacuole which is made distinctly visible by treating with Lugol's solution. 

 When stained two typical nuclei are recognized in the sporoplasm. Dimen- 

 sions of the fresh spores: length excluding tail 13.5 to 15/x, breadth 8 to 

 9/x, thickness 6 to 1 .hn, length of tail 30 to 35/x, often up to 40)Lt, polar 

 capsule 5 to (>fx by 2)n, length of polar filament 40ai. 



The characters of the genus are described on page 59. 

 Type and only species: Hoferellus cyprini Doflein. 



