391] STUDIES ON MYXOSPORIDIA—KUDO 153 



MYXOBOLUS NODULARIS Southwell et Prashad 



[Figs. 479 and 480] 



1918 Myxobolus nodularis Southwell and Prashad 1918 : 347 



Habitat: In the muscles of Rasbora daniconius occurring in two fish 

 on the sides, and in another as a globular cyst near the anus; Mirpur, 

 Dacca (June). Type specimens, numbered P 52/1. 



Vegetative form : Cysts rounded or slightly elongated, varying in length 

 3.5 to 3.8mm. and 2.3 to 2.8mm. in width. Creamy yellow in color, in one 

 case appearing blackish owing to the large number of black granules 

 scattered in its surface. 



Spore: Ovoidal. Sutural ridge very wide (about 1/5 thickness of the 

 spore). Two polar capsules of equal size, which show coiled polar filaments 

 clearly. Dimensions: length 9/i, breadth 7.2/x, length of polar capsule 

 3.4/i, that of polar filament 18.3/i. 



MYXOBOLUS HYLAE Johnston et Bancroft 



Habitat: In the testes, vasa efferentia and oviducts of Hyla aurea; 

 Sidney, Australia (April, other months not mentioned). Fletcher observed 

 the parasites also in the urinary bladder of both sexes, which fact was not 

 confirmed by Johnston and Bancroft on account of the scarcity of the 

 material. The latter authors could not infect Hyla caerulea by feeding 

 infected testes or the cysts, giving the conclusion that the parasite is 

 specific to H. aurea. The male is more often attacked by the parasite 

 than the female. The infected animal appeared sickly and emaciated. 

 As to the infection in kidneys, they write as follows: In one male specimen 

 both testes and both kidneys were affected, and the upper parts of the 

 ureters adjacent to the kidneys were swollen and milky in appearance. 

 In another, in addition to the testes, the adjacent kidney and mesentery 

 were attacked. No spores have yet been detected by them in sections of 

 the kidney tubuies. 



Vegetative form: Johnston and Bancroft describe as follows: 

 Cysts: in male, either imbedded in the tissue or may project freely into 

 the coelom of the testes; in female, lying between the layers of the wall, 

 being projected into the lumen of the oviduct. Size from those of micro- 

 scopic dimensions up to 2 to 3mm. in diameter. In sections, the protoplasm 



