ZOOLOGY AND BOTANY, MICROSCOPY. ETC. 177 



somewhat resembling H. ziemanni in one. The guinea fowl {Numula 

 pUhrhyncha) appears to be very liable to blood-parasites ; in one case 

 Halteridium, an Hmnamceba, and two types of Filaria, as well as an 

 undetermined parasite, were found on a single slide. 



New Species of AmcEbidium.* — ^E. Chatton describes Amce.bidium 

 recUcola sp. n., a commensal of Daphnia. He distinguishes it from 

 A. parasiticum in the following diagnoses. A. parasitkum is very 

 variable in form and size ; it is an external commensal of fresh-water 

 Arthropods, and may incidentally develop in the rectum of Cladocera. 

 It has fusiform spores, slightly arcuate, varying in length from 15- 

 30 )U.. A. recUcola sp. n. is thick and bent at base like a pistol-butt, is 

 a commensal in the rectum of Daphnia and other Cladocera ; its spores 

 are cylindrical, 8-12/*. 



Culture of Trypanosoma rotatorium.t — G. Bouet gives an account 

 of the morphology of this parasite free in the blood of Rana esculenta 

 and in cultures. In the latter, auto-agglutination of young forms 

 occurred, and there appeared a large number of examples with highly 

 refringent granules. Others of a pearly opalescent aspect appeared in 

 the water of condensation. The vitality of the cultures was considerable ; 

 in one, trypanosomes lived for five months, but this was exceptional. 

 Various differences between the natural and cultural forms are noted. 

 In particular the latter were much smaller than the natural examples. 

 Attempts at inoculation of Rana, Bufo, and Pelohates were unsuccessful. 



Nucleus of Trypanosomes. J — Ronald Ross and J. E. S. Moore 

 have observed that on colouring liquid blood containing trypanosomes 

 {T. brucei and T. equiperdum) by various stains, especially the nuclear 

 stains (such as basic fuchsin and thionine), the deeply-stained body does 

 not coincide with that shown by the various modifications of the 

 Romanowsky-Ziemann method as usually applied to dried films, but 

 consists of a much smaller sphere within the latter. It would appear, 

 therefore, that the smaller sphere is the true chromatin portion of the 

 nucleus. This observation tends to re-open the cytological interpreta- 

 tion of trypanosomes. 



Yellow Fever in Chimpanzee.§ — H.Wolferstan Thomas notes that 

 some Stegomyia fasclata, allowed to feed on two cases of yellow fever, 

 were allowed thereafter to feed on a chimpanzee, which developed a 

 benign attack of yellow fever and recovered. Mosquitos were allowed 

 to feed on the chimpanzee during the infective period, and these will be 

 in due course applied to a " non-immune " man to complete the cycle of 

 ' man-chimpanzee-man." 



Trypanosoma gambiense in Tsetse Fly.|l — E. A. Minchin reports 

 that in Uganda, experiment and observation have shown that Trypano- 

 soma gcunbiense does not pass through a developmental cycle in the 



* Arch. Zool. Exp6r., Notes et Revue, No. 2, xxxv. (1906) pp. xxxiii.-xxxviii. 



(4 figs.). 



t Ann. Inst. Pasteur, xx. (1906) pp 564-77 ( 1 pi. and 2 figs.). 

 X Brit. Med. Journ., Jan. 1907, p. 138. § Loc. cit. 



II Nature, Ixxv. No. 1933 (1906) pp. 56-9 (3 figs.). 



April 17th, 1907 N 



