ZOOLOGY AXD BOTANY, MICROSCOPY, ETC, 247 



to admit the canula into the lamina of the segmentary canals. The in- 

 jection is carried out under water, and when over the piece is immersed 

 in 90 p.c. alcohol to set the metagelatin. It is then stained eii tnasse 

 with alum-carmin, after which the preparation is carefully stripped of 

 adherent tissues and then cleared up in oil of cloves and mounted in 

 balsam. 



By means of Schiefferdecker's corrosion method, casts of the renal 

 cavities were obtained. This method consists in filling the renal canali- 

 culi with celloidin coloured with asphalte, and then dissolving ofif the 

 tissues with hydrochloric acid. 



Soft Injection Mass for Glycerin Preparations.* — C. Skoda finds 

 that hollow viscera, such as intestine, when preserved in glycerin are 

 susceptible of making excellent specimens when injected. The material 

 is immersed in glycerin, to which ^ of a 2 p.c. formal-hydrate solution 

 (1 part commercial formalin and 1 of water). After 6-S days the 

 specimen is taken out and most of the glycerin removed by squeezing ; 

 it is then placed on a dry cloth and rolled up. In this sausage-like state 

 it is placed between two boards, which are either tied together or pressed 

 together by means of a weight. In two days time the specimen is ready 

 for its further treatment. Should the specimen be too dark, it may be 

 bleached for 12-4:« hours in i- p.c. formol solution, to which -jV of a 

 3 p.c. peroxide of hydrogen solution is added. This bleaching is to be 

 effected before the immersion in the glycerin-formalin mixture. 



The injection mass f consists of isinglass, white dextrin, and a pig- 

 ment, cinnabar for arteries, ultramarine-blue for veins, in the proportion 

 of 2-1, 0*5-l. To this mass, when thoroughly incorporated by rubbing 

 up in a mortar, so much water is added as will impart a honey-like con- 

 sistence, it may then be injected into the vessels by means of a Teich- 

 mann's syringe. The injection is best made under water. The specimen 

 is preserved in glycerin. For further minute details the original should 

 be consulted. 



Staining Medullary Sheath of Nerves. | — W. Stoeltzner communi- 

 cates the following simple method. The tissue is fixed on formalin and 

 imbedded in celloidin. The section is mordanted for 5 minutes in the 

 officinal liquor ferri sesquichlorati. After a wash in distilled water, it 

 is immersed for at least 10 minutes in 0-5 p.c. aqueous haematoxylin 

 solution. The now black stained section is differentiated in Weigert's 

 ferri-cyanide-borax solution or in the iron-chloride mordant mixture. 



Injecting the Arteriolse rectse of Mammalian Kidney.§— Gr. C. 

 Huber used a modification of Krassuskaja's injection mass. It was com- 

 posed of photoxylin 30 grm., camphor 20 grm., aceton 600 c.cm., and 

 was made by adding 0*5 grm. alkanin dissolved in 20 c.cm. aceton to 

 80 c.cm. of the above described mass. About 10 minutes after injection 

 the organ was cut up into pieces, and these were placed for 24 hours in 

 75 p.c. hydrochloric acid in which the tissues are so macerated that they 

 may be readily washed away with water, leaving a cast of the blood 



* Anat. Anzeig., xxix. (1906) pp. 602-5 (3 figs), 

 t See this Journal, 1906, p. 739. 

 + Zeitschr. wis.;;. Mikroslv., xxiii. (1906) p. 329. 

 § Brit. Med. Journ., 1906, ii. p. 1700. 



