ZOOLOGY AND BOTANY, MlGROSGOrY. KTU. 



Ill 



Cultivating the Streptococcus of Impetigo contagiosa.* — J. E. H. 



Roberts has introduced a method to demonstrate this organism without 

 the aid of differentiating conditions of cultivation. The cultures are 

 made from a drop of the serum which oozes from the raw surface of the 

 base of the lesion, after the crusts have been removed and the raw 

 surface has been rubbed clean with alcohol. The drop of serum is put 

 into the condensation water of the culture tube, which is then allowed 

 to flow over the surface of the medium, and the tube is incubated for 

 24 hours. The fine streptococcus colonies are then easily distinguished 

 from those of staphylococcus. Pure subcultures are thus easily obtained, 



(2) Preparing^ Objects. 



New Fixing and Washing Vessel.f — A. Breckner describes a piece 

 of apparatus (fig. 27) devised by K. Kreigbaum for the fixing of tissues 

 for microscopical purposes. This is a glass vessel with a capacity of 

 about 250 c.cm., provided with a cover, and capable of being emptied 

 through a stop-cock. A glass funnel is fitted inside this, which can 

 hold the material to l)e fixed in its upper wider portion. Then when 

 the apparatus is filled with fixative fluid all precipitates and heavy salts 



Fig. 27. 



fall down the narrow part of the funnel to the bottom of the apparatus, 

 and can be drawn off by means of the tap. It thus becomes unnecessary 

 to change the fixing fluid entirely, but the small quantities drawn off 

 may be replaced from time to time. Tlie tissues rest all the time in 

 fresh fluid. In theory the apparatus is excellent, but practically it is 

 more suited for the washing than the fixing of material. 



ZiKES, H.— Die Fixierung und Farbung der Hefen. 



[Af'review and recapitulation of numerous methods of fixing and staining 

 yeasts.] Centralbl. Bakt., 2te Abt., xxxi. (1911) pp. 507-34. 



* Med. Review, xiv. (1911) pp. 81-7 (12 figs.). 



t Zeitschr. wiss. Mikrosk., xxvii. (1911) pp. 504-6 (2 figs.). 



