IIOTSOX. — CULTUHK STIDIKS OF FrN(iI. 251 



with biilhils l)y Bainier may also he nielunosporous and will he re- 

 ferred to later on. 



Melanospora papillata n. sp. 

 Plate 2, Figures 1-26. 



Perithecia scattered or gregarious, superficial, niemhranous, semi- 

 translucent, straw-coloreil to light hrown, glohose to pyriform, 

 350-450 fx X 400-500 fx, papilla surmounted hy erect, somewhat 

 divergent, continuous setae, 100-170 jj. in length; primordium a 

 group of one or more intercalary cells; ascospores asymmetrical, 

 somewhat crescent-shaped 10 X 25 n, yellowish brown becoming 

 black; conidia abundant, hyaline, spherical to ovoid, on flask-shaped 

 sterigmata; bulbils yellowish brown, irregular in outline, 50-CO /x in 

 fliameter, sometimes considerably more than this. 



On Live Oak bark {Quercus ogrifolia Nee) from Pomona, Cali- 

 fornia. 



A pure culture of this species was easily obtained by making a 

 transfer of the ascospores in the manner already described, on rich 

 nutrients, fairly soft,. with about 20 gm. of agar to the litre, and both 

 perithecia and bulbils were produced abundantly. On substrata, 

 however, poorly supplied with nutrient material, such as sterilized 

 agar-agar, or even on a medium well supplied Avith food material if 

 made very hard (about 40-50 gm. of agar to the litre) the bulbils are 

 very sparingly produced if at all, the mycelium is quite inconspicuous 

 and the perithecia appear scattered over the surface more or less 

 al)undantly. In its capacit\^ to retain its power of producing peri- 

 thecia this species resembles M. cervicula, while it is in sharp contrast 

 to some other melanosporous forms studied in which, after long 

 artificial cultivation the bulbils tend to become the dominant mode 

 of reproduction and the perithecia are produced sparingly if at all. 



The bulbils. The hyphae, winch vary in diameter from 4-7 //, are 

 hyaline, with numerous oil globules and prominent cross walls, and 

 are usually very scantily developed. The bulbils make their appear- 

 ance as small straws-colored bodies scattered somcAvhat sparingly 

 and usually in small patches over the surface of the substratum. 

 In the process of development, which was carefully followed in \'an 

 Tieghcm cells and in pure cultures in test-tubes, hyphae divide up 

 into short intercalary almost isodiametric cells, one or more of which 

 enlarge (Figure 1, Plate 2) while the contents becomes den.sely granu- 

 lar and filled with oil globules. At this stage these enlarged cells are 



