Bacteria: Recombination {II) 



351 



FIGURE 38-2. Replica-plating (shown diagrammaticully) to detect 

 genetic recombination in E. coli. A completely prototrophic 

 recombinant is found at 12 o'' clock in all replicas. A recombinant 

 for both Pa and C is found at 3 o'clock on replicas 5 and 6. Replica 

 J has a clone growing at 9 o'clock which may be due either to 

 recombination or to mutation to T+. 



T L B.B Pa C T L B, B Pa C 



MASTER PLATE 



T L B,B Pa C ^Medium Contair 



SIX REPLICAS 



T L B.B Pa C 



T L B,B Pa C 



T L B,B Pa C 



T L B, B Pa C 



T L B,B Pa C 



T L B,B Pa C 



