Bacteria: Recombination (F) 



381 



Jacob, F., and WoUman, E. L., "Genetic Aspects of Lysogeny," pp. 468-500 in A Symposium 

 on the Chemical Basis of Heredity, McElroy, W. D., and Glass, B. (Eds.), Baltimore, 

 The Johns Hopkins Press, 1957. 



Kaiser, A. D., and Hogness, D. S., "The Transformation of Escherichia Coli with Deoxy- 

 ribonucleic Acid Isolated from Bacteriophage Xdg," J. Mol. Biol., 2:392-415, 1960. 



Morse, M. L„ Lederberg, E. M., and Lederberg, J., "Transduction in Escherichia Coli K-12," 

 Genetics, 41:142-156, 1956; reprinted in Papers on Bacterial Genetics, Adelberg, 

 E. A. (Ed.), Boston, Little, Brown, 1960, pp. 209-223. 



Ozeki, H., "Abortive Transduction in Purine-Requiring Mutants of Salmonella Typhi- 

 murium," Carnegie Inst. Wash. Publ. 612, Genetic Studies with Bacteria, 97-106, 

 1956; reprinted in Papers on Bacterial Genetics, Adelberg, E. A. (Ed.), Boston, 

 Little, Brown, 1960, pp. 230-238. 



WoUman, E. L., and Jacob, P., "Lysogeny and Genetic 

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 Stent, G. S. (Ed.), Boston, Little, Brown, 1960, pp. 

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Zinder, N. D., "'Transduction' in Bacteria," Scient. Amer., 

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Zinder, N. D., and Lederberg, J., "Genetic Exchange in 

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QUESTIONS FOR DISCUSSION 



41.1. How would you define the term provirus? How do 

 the terms merozygote and heterogenote differ? How 

 would you define a homogenotel 



41.2. What characteristics are conferred upon a host cell 

 infected by a nontransducing temperate phage which 

 (1) becomes a prophage? (2) does not become a 

 prophage? 



41.3. How would you proceed to prove that there is only one exogenote in a microcolony 

 of Salmonella produced following abortive transduction? 



41.4. Discuss the statement: "Temperate phage has chromosomal memory, and the 

 chromosome has temperate phage memory." 



41.5. F particles are known which carry the prophage of X as "memory." How could 

 you prove the existence of such a particle? 



41.6. Describe the procedure and genotypes you might use in demonstrating that E. coli 

 can undergo genetic transformation with respect to Gal. 



41.7. Is there any reason for believing that the close linkage of genes with related effects 

 might be more advantageous in microorganisms than in higher organisms? Explain. 



41.8. List the different ways that the Gal locus in E. coli may undergo recombination. 



41.9. Would you say that temperate phages are good or bad for bacteria? Explain. 



Give an example of this process 



Norton D. Zinder, 

 about 1954. 



41.10. How would you define sex-duction or F-ductionI 

 in E. coli. 



41.11. Is a cell which has presumably stopped undergoing mutation, genetic recombination, 

 and self-replication of its DNA still considered to contain genetic material? Explain. 



