348 J. B. SCRIVEN ON PREPARATION OF SERIAL SECTIONS OF INSECTS. 



spirit for *' ten minutes," is replaced by an almost instantaneous 

 flooding with absolute alcohol to remove the benzoline ; (5) air 

 cavities in the specimen are avoided by using the exhauster ; 

 (6) the danger of vacuoles is eliminated ; (7) the use of an 

 adhesive material and of No. 4 bath hefwe staining * is rendered 

 unnecessary ; (8) wrinkles are obliterated. 



Against these obvious gains must be put the time, amounting 

 to about 2 5 hours, spent in stretching and fixing. Still, con- 

 siderable benefit remains in the shortening of the aggregate time 

 occupied by all the proceedings, in the larger percentage of success, 

 and the more satisfactory results. 



* The object of using Dr. Lowiie's No. 4 bath at this stage of the 

 proceedings was to prevent the staining of the adhesive material. 



Jowm. Quekett Microscopical Club, Ser. 2, Vol. VIII., No. 51, November 1902. 



