INTERACTIONS BETWEEN VENOM AND ANTIVENIN 



259 



With another preparation of Calmette antivenin, much more powerful, and 

 4 c.c. of which neutrahzed 0.003 g^^- of the venom, the results shown in 

 the third column of table 36 were obtained. 



Figure 12 shows the results of experiments with the first antivenin and 

 Figure 13 those of the second antivenin. 



The results of partial neutralization of cobra ha^molysin by the antivenin 

 are as follows: For the test 8 c.c. of i per cent suspension of washed horse 

 corpuscles (each liter containing 8 c.c. of o.oi n lecithin) in each tube were 

 used. The test dose of venom was i c.c. of o.i per cent cobra venom. Two 

 preparations of antivenin were used (table 37 and fig. 14). 



Table 37. 



100 



0.1 0.2 03 0.4 0.5 



o Neutralization with antivenin I. 

 a Neutralization with antivenin II. 



Fig. 14. — Cobralysin antilysin. Horse blood with 

 lecithin. 



0.6 



WATER-MOCCASIN-VENOM ANTIVENIN. 



With this venom the partial neutralization with its .specific antivenin gave 

 a singular result. When 2 c.c. of the antivenin were added to 10 minimal 

 lethal doses of the venom (0.012 gm.) the mixture contained about 6 minimal 

 lethal doses, but with 8, to, 20, and 40 c.c. there was no complete neutralization. 

 Madsen and Noguchi left this phenomenon to a later investigation, not 

 having enough material for further w'ork at that time. 



The neutralization of the haemolytic component of water-moccasin venom 

 with the specific antivenin gave the following results : 8 c.c. 5 per cent suspen- 

 sion of dog blood in 0.9 per cent NaCl were used for the test reaction. 



