THE STUDIES ON THE CELL NUCLEAR 



NUCLEPROTEINS WITH THE AID 



OF PHENOL FRACTIONATION PROCEDURE 



G. P. GEORGIEV 



A. N. Severtzov Institute of Animal Morphology. 

 Academy of Sciences of the USSR, Lenin avenue, 33, Moscow W 71 USSR 



In the course of studying of somefeatures of phénol deproteinization mech- 

 anism it was shown that the resuit of phénol treatment dépends on the 

 type of protein part of nucleoprotein complex and on Ihe nature of the bond 

 between nucleic acid and protein. After the treatment with phenol/014 M 

 NaCl the major part of cytoplasmic RNA and nuclear sap RNA pass into 

 the aqueous phase. DNA and nucleolar and residual chromosomal RNAs 

 remain in the layer forming ou the water phénol boundary. In this inter- 

 mediate layer cell nuciei exist, which were further puriphied. Phenolic 

 nuclei contain DNA, RNA and protein. Their composition is similar to the 

 composition of nucleolar-chromosomal complex. The RNA content of phe- 

 nolic nuclei correlates with the development of nucleolar apparatus of the 

 parrent tissue. The treatment with phénol pH 7-3— 7'4/0'14 M NaCl libér- 

 âtes DNA into the aqueous phase. The method for DNA isolation is based 

 on this procédure. RNA of phenolic nuclei may be separated with phénol 

 pH 6/H2O or with phénol pH 7-3— 7-4/014 M NaCl on two fractions: ex- 

 tractable RNA-E and non-extractable RNA-N. It was shown that incorpo- 

 ration of P'^ in vitro into RNA-N of Ehrlich ascites carcinoma cells is 

 3—5 times as higher as into RNA-E and 40—80 times as higher as into 

 high-polimeric cytoplasmic RNA (1 h incubation). 



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