CYTOCHEMICAL 



AND ELFXTRON MICROSCOPE STUDIES 



OF FROG ERYTHROCYTES* 



M. A. LESSLER** 



Department of Physiology 

 The Ohio State University, Columbas, Ohio 



Although many différent variations in technique for the démonstration of 

 dehydrogenase activity by tetrazoliums were used, no staining of frog 

 erythrocytes was observed. The white cells, however, did show some 

 stained granules. Neutral red granules were observed in red cells, but no 

 mitochondrial-like éléments could be demonstrated with the Janus Green B 

 technique. Thin-sectioned électron microscope préparations showed the 

 présence of erythrocyte mitochondria. Histochemical techniques were 

 positive for deoxyribose nucleic acid and protein, but ribonucleic acid, 

 mucopolysaccharides, and glycogen were not demonstrable with the 

 techniques used. 



- 51 - 



