ULTRAVIOLET FLUORESCENCE MICROSCOPY 

 AS A NEW FIELD OF HISTOCHEMISTRY 



M. N. MEISSEL, E. M. BRUMBERG, T. A. GRIGORJEVÂ, 

 L J. BARSKI and A. W. GUTKINA 



Institute of Biophysics, 

 Academy of Sciences of USSR, Moscow 



Fluorescence raicroscopy in visible spectral région bas already occupied 

 a definite place among important histochemical methods. As it is well 

 known it requires in most cases the usage of fluorescent stains for 

 revealing spécifie substances in cells and tissues. 



Fluorescence microscopy in ultraviolet région spectral, which does 

 not need any fluorocbromes, allows to detect by photographie means 

 with subséquent colour transformation of the images, the ultraviolet 

 autofluorescence of cells, tissues and other biological objects. The 

 spécifie nature of this autofluorescence dépends on the peculiarities- of 

 their chemical composition and physico-chemical state. 



The systematic investigations of différent tissues and organs showed 

 that many of them possess strongly pronounced emd spécifie ultraviolet 



autofluorescence. 



We hâve corne to conclusion that ultraviolet fluorescence microscopy 

 appears to be of great importance for the histochemical studies of such 

 objects as muscle, collagenes, and some secreting cells. 



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