SECTION III. HISTOCHIMIE EVOLUTIVE 



DEVELOPMENTAL HISTOCHEMISTRY: 



INTRODUCTION OF NEW METHODS FOR LIGHT 



AND ELECTRON MICROSCOPY BY THE DESIGN 



AND PREPARATION OF APPROPRIATE REAGENTS 



AND SUBSTRATES 



A. SELIGMAN 



The historical features of the évolution to the présent state of perfection 

 is briefly summarized for acid phosphatase, esterase, leucine aminopepti- 

 dase, succinic dehydrogenase and cytochrome oxidase. It was shown that 

 the major advance in methodology was accomplished by design and syn- 

 thesis of appropriate reagents to fulfill the stringent requirements of mor- 

 phological histochemistry and cytocheraistry. 



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