HISTOLOGICAL AND PHYSIOCHEMICAL PARALLELS 



IN MICE FOLLOWING HETEROLOGOUS 



BONE MARROW TRANSPLANTATION 



G. C. ROYAL, JR. 



Department of Biology and Gladys W. Royal, Department of Chemistry, 

 The Agricultural and Technical Collège of North Carolina 



Alkaline phosphatase, an enzyme specifically présent in rat bone marrow 

 cells, can serve as an index to differentiate between injections and 

 successful transplants of rat bone marrow in mice. Normal (B x D)F, 

 females, after exposure to 900r and protective amounts of rat bone marrow 

 were sacrificed. Histological studies of blood and spleen were made. 

 Seven organs, liver, lung, spleen, kidney and brain were measured for 

 tissue weight and serotonin level. Lymph nodes and spleens were further 

 tested for hemagglutination with rat globulin. Ultra-violet and electropho- 

 retic pattems of sera were obtained. Initial redtiction in serotonin level; 

 positive hemagglutination; increased subcutaneous response to vasocon- 

 stricting agents; altération in ultraviolet absorption and electrophoretic 

 patterns are among the findings that parallel the positive alkaline phos- 

 phatase tests. 



TTiis work bas been supported by U. S. Atomic Ejiergy Commission Contract 

 No. AT (40 - 1) 2399. 



- 128 - 



