THE HISTOCHEMISTRY 

 OF RESPIRATORY ENZYMES 

 IN CARCINOGENESIS 

 I 



J. CHAYEN, A. A. SILCOX, E. K. AVES and A. L. E. BARRON 



Department of Pathology, Royal Collège of Surgeons, 

 Lincoln's Inn Fields, London 



In expérimental liver carcinogenesis, gross biochemical estimation would 

 suggest a seccessive loss of succinic dehydrogenase activity per unit 

 weight of tissue. Whether such changes are due to effects within the 

 parenchyma cells or eire due to prolifération of cells of other origin which 

 normally hâve a low dehydrogenase activity, can be demonstrated only 

 by histochemical investigations. Such a study will be reported; the 

 tissue is prepared by controUed température freeze-sectioning and endo- 

 genous respiration, endogenous and total succinic dehydrogenase are 

 compared. The significance of histochemically demonstrable différences 

 in respiratory enzymes of bile duct and parenchyma cells will be consid- 

 ered in relation to carcinogenesis. 



In fully tarmed tumours the enzymic activity in différent régions 

 varies. To test the activity of respiratory enzymes in active tumour 

 material, recourse has been made to secondary growths of liver cells 

 in lung. 



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