74 



MICROSOMAL PARTICLES 



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17 



The incorporation of glycine-1-C 14 into the adenine and glycine of rat liver RNP, 

 and into "supernatant" and serum proteins (see text). 



Fig. 4. 



at 15 minutes and then dropped off. The glycine of the liver supernatant pro- 

 teins, plus the microsomal lipoproteins soluble in deoxycholate, reached a maxi- 

 mum between 1 and 2 hours, and then fell. The glycine of the serum proteins 

 increased very slowly, and was highest at 17 hours. Similar results have been 

 obtained with labeled adenine and methionine as tracers. 



The lower row shows the results obtained on rats pretreated with bovine 

 growth hormone, 410 [\g per rat per day, for 2 weeks. There was no effect on 

 the RNP adenine, but the effects on the three protein fractions were marked. 

 Everything seemed to have been speeded up. The nucleoprotein glycine ac- 

 tivity dropped more rapidly; the liver supernatant activity rose more steeply; 

 and the serum proteins apparently reached a maximum at some time before 17 

 hours, and then declined again. 



These results illustrate, first, the lack of correspondence in the extents of 

 isotope incorporation into the RNA and the total protein glycine of the RNP, 

 and, second, the effect of growth hormone, which speeded up the incorporation 

 of glycine into protein without any change in the rate at which the particle 

 RNA became labeled. From these observations we conclude that, at least in 

 rat liver, the total RNA of the RNP cannot be functioning as an active template. 



