CHAPTER IV 



MICRURGY 



The microdissection of living cells was first done a century ago. 

 The pioneer workers on living matter, von Mohl, Dujardin, 

 Johannes Muller,and vonBaer, 

 dissected cells with great inge- 

 nuity. Purkinje (1844) sug- 

 gested microdissection, and 

 Chabry (1877) carried it out. 

 Later came the micromanipula- 

 tive work of W. Roux, E. B. 

 Wilson, J. Loeb, and others, 

 which had to do with relatively 

 simple dissections, involving 

 such operations as the punctur- 

 ing of an egg and the separation 

 of the cells (blastomeres) of an 

 embryo. H. D. Schmidt as 

 early as 1859, Herlitzka in 

 1895, Kopsch in 1900, and 

 McClendon in 1905 carried on 

 microdissection with mechani- 

 cally controlled needles, but 

 the work ended with their few 

 experiments. It was not from oK""n^Z i> i t ^ ^;„.^ 



^ , Fig. 35. — The Barlui-Kite micro- 



the work of the pioneer micro- dissection instrument. In this photo- 



dissectionists that inicrurgy graph, the instrument is equipped with 



an electromagnet for attractmg minute 

 directly arose to its present nickel particles which have been in- 

 state of perfection. ^^^^^ ^ft" the Uving cell, and which 

 ^ when attracted, give an indication ot 

 The task of obtaining pure the viscosity and the elasticity of the 



cultures is one with which the protoplasm The two fine glass needle 



points and the heavier tip ol the 

 bacteriologist has always had magnet core are to be seen within the 



to deal. The condition toward f^l^tly ^^>ble glass chamber under the 



microscope lens. 



which he strives is a pure 



culture started from a single bacterium, for then not only is 



47 



