94 



MARTIN G. LARRABEE AND PAUL HOROWICZ 



30CH 



O 



5 



I 



2 

 o 



i i i 



12 3 



HOURS 



Fig. 4. Record of rate of oxygen consumption by a rat ganglion, obtained with the 

 respirometer shown in Figure 3. Sodium azide (0. 5 raM) was added at the arrow. Ex- 

 planation in text. 



of sufficient concentrations of azide. Two later calibrations show that the sensi- 

 tivity of the system had not changed. 



Glucose and Lactate 



To measure exchanges of glucose and lactate, a ganglion was supported in 

 the gas phase above a tiny glass vessel, so that it was exposed to moist 95% 

 0> , 5% C0 2 (Fig. 5). By raising the vessel, the ganglion was dipped into 

 the solution for a fraction of each minute, in order to supply glucose and re- 



GANGLI0N 



SOLUTION 



*=— GAS IN 



GLASS 

 VESSEL 



|*-5mm -a4 



Fig. 5. Rat ganglion mounted for measurement of glucose uptake and lactate pro- 

 duction. The glass vessel was raised to immerse the ganglion for about 15 seconds every 

 minute. (Edwards and Larrabee, 1955). 



