114 



-03S 



c 



S03 



O" 







we have a tri-negative ion which still binds strongly to albumin. However, the 

 compound containing a positive group, compound (b), is still a negative ion and 

 if the bindings here are due to the negative containing charge in all cases we 

 would expect a continued binding, even of the substances of the positive ion, 

 since it still has the same configuration about the negative charges as in sub- 

 stance (a). Actually, we find that the presence of a positive ion group in com- 

 pound (b) decreases its ability to bind to albumin tremendously, although we 

 have the same negative charge configuration as in the case of the tightly bound 

 non-polar substance. Thus, the effect of the positive ion appears to be more com- 

 plex in certain cases than in the situation you mentioned where the compounds 

 you studied did not bind simply because there was apparently no receptor site 

 for it. 



Professor Klotz: Evidently, I should have shown another illustration. 

 Suppose we are measuring the extent of binding against the concentration of 



z 

 o 



m 



LOG OF CONG. 



