Plate II. Comparison of freeze-drying and chemical fixation. Both figures 

 are ultraviolet photographs of Walker rat sarcoma, taken by R. King; 

 2570 A.U.; 2-mm. quartz objective. Figure A shows fixation by Carnoy's 

 method, and Fig. B by freeze-drying. Note that with chemical fixation 

 there is a halo surrounding the metaphase plate, and the intermitotic nuclei 



