D 



Plate VIII. Walker rat sarcoma 4 days after treatment by nitrogen mus- 

 tard. Figures A and C, Feulgen; fixative, Carnoy. Figures B and D, 

 alkaline phosphatase by Gomori-Takamatsu method; fixative, 80 percent 

 alcohol; incubation time, 120 minutes. Note parallelism between distri- 

 bution of phosphatase and deoxy nucleic acid. Figures A and B show cells 

 in which spindle formation has failed, so that metaphase chromosomes are 

 scattered throughout the cytoplasm. Figures B and D show pycnotic 



degeneration. 



