48 



STUDIES ON ALKALINE PHOSPHATASE 



TABLE V 



Distribution of alkaline phosphatase in sections after fixation in various 

 media, and after freeze-drying. ++ indicates gross shrinkage; +, some 

 shrinkage; 0, no change; — , some swelling; =, gross swelling. 



Shrink- 



Fixative 



None 



Freeze-drying 

 Ethyl alcohol 80% 

 Formaldehyde 4% + so- 

 dium chloride 1% 

 Acetone 

 Pyridine 



Pyridine 70% 



Pyridine 25% in alcohol 

 80% 



Calcium chloride 1% in 

 pyridine 70% 



Formaldehyde 4% + cal- 

 cium chloride 1% 



Saturated picric -f- formal- 

 dehyde 4% 



Ethyl alcohol 65% + chlo- 

 roform 35% 



Pyridine 70% + formalde- 

 hyde 4% 



Pyridine 20% + ethyl al- 

 cohol 70% + formalde- 

 hyde 4% 



Alcohol 60% + chloroform 

 30% + formaldehyde 



Formaldehyde 4% + ace- 

 tic acid 1% 



Formaldehyde 4% + ace- 

 tic acid 5% 



Ethyl alcohol 80% + ace- 

 tic acid 1% 



Pyridine 25% + picric 

 (sat. soln.) 75% 



Saturated aqueous ammo- 

 nium sulfate followed by 

 alcohol 80% 



Saturated aqueous calcium 

 chloride followed by al- 

 cohol 80% 



Saturated aqueous magne- 

 sium sulfate followed by 

 alcohol 80% 















+ 



+ + 

 + + 



Distribution of Enzyme 



Mainly in cortical tu- 

 bules: smeared appear- 

 ance 

 Brush borders and nuclei 

 Brush borders and nuclei 

 Brush borders and nuclei 



Brush borders and nuclei 

 Brush borders and nuclei 



Brush borders and nuclei 

 Brush borders and nuclei 



Brush borders and nuclei 



Brush borders and nuclei 



Enzyme destroyed 



Enzyme spread all over 

 proximal tubules 



Very poor Enzyme spread over 



whole section 

 Very good Brush borders and nuclei 



Poor 



Brush borders and nuclei; 

 some spreading into 

 cytoplasm 

 Very good Brush borders and nuclei 



Good 

 Fair 



Fair 

 Fair 



Poor 



Fair 



Enzyme destroyed 

 Brush borders and nuclei 



Brush borders and nuclei 

 Brush borders and nuclei 



Brush borders and nuclei 



Brush borders and nuclei 



