194 



INFLUENCE OF TEMPERATURE ON BIOLOGICAL SYSTEMS 



Fig. 10. Responses of B fibers at low temperatures. Four different experiments (A, B, 

 C, D). 



Column A: 4.2°C. Record 1 is the control. A solution with no NaCl but with TMA 

 was applied and records 2-7 were taken at 3', 7', 8', 9', 10' and 11'. At 12' a solution 

 with 11 mM NaCl (with TMA) was added. Responses 8 and 9 were taken at 13' and 

 30'. Ringer wash occurred at 31' and recovery for 32' (record 10) is shown. All records 

 have 60-cycle time line on lower beam. Record 11 shows this time line on upper beam. 



Column B: 3.2°C. Record 12 is the control. A solution without NaCl (with TMA) 

 was added and records 13-18 were obtained at 11', 15', 17', 19', 20' and 21'. At 22' 

 a solution with 11 mM NaCl (with TMA) was applied. Records 19-21 are shown for 

 23', 27' and 35'. Ringer wash took place at 36' and recovery for 38' (22) is given. 



Column C: 5.2°C. Record 23 is the control. The solution without NaCl (with TMA) 

 was added and the responses (records 24-27) are given for 11', 17', 19', and 25' . At 

 26' a solution with 11 mM NaCl (with TMA) was applied. The responses (records 

 28-32) are listed for 27', 29', 32', 35' and 50'. Ringer wash occurred at 51' and recovery 

 is shown for 53' (33). 



Column D: 5.2°C. Continuation of column C. Record 33 is the control. A solution 

 without NaCl but with choline chloride was added at 61' and the responses (records 

 34-37) are given for 63', 75', 77' and 78'. At 79' a solution with 11 mM NaCl (with 

 choline chloride) was applied. Recovery was allowed to proceed to its maximum and 



