vi Table of Contents 



Adrenalin 1^^ 



The EnterochromaflRn Substance 124 



Sulfhydryl (Thiol) Groups 128 



E. Various Unclassified Organic Substances .... 130 



F. Pigments 132 



VIII. Enzymes 1^*^ 



Preparation of Tissues for Enzymatic Reactions . . . 138 



Histochemical Reactions for Enzymes 141 



1) Oxidative Enzymes 1^0 



a) Dehydrogenases 1^0 



b) Oxidases 1^^ 



c) Peroxidases 1"2 



Appendix: Unna's Reduktionsorte and Sauerstofforte . 165 



2) Hydrolytic Enzymes 167 



Methods for Hydrolytic Enzymes in General . . . 167 



Phosphatases 172 



Alkaline Phosphatases • • 175 



5-Nucleotidase 186 



Lecithinase (Phospholipase) 187 



Acid Phosphatase 189 



Phosphamidase • • 1^4 



Phosphorylase 197 



Zymohexase (aldolase) 197 



Sulfatase 198 



Esterases 200 



I. AHesterases 202 



II. Cholinesterases 208 



The Results of Histochemical Methods for Esterases . 212 



/3-Glucuronidase 216 



Carbonic Anhydrase 218 



Urease 219 



Appendix: Buffers for Use in Histochemistry . . . 219 



ADDITIONAL REFERENCES 



Additional References 225 



INDEXES 



Author Index 253 



Subject Index 266 



