TABLE OF CONTENTS 



Introduction 1 



PART I. HISTOCHEMICAL METHODS 

 IN GENERAL 



I. The Nature of the Processes of Identification in 



Histochemistry 7 



II. The Special Features of Histochemical Methods . . 10 



III. The Histochemical Routine 14 



IV. Controls To Prove Validity of Technique . .' . . 17 

 V. Quantitation in Histochemistry 20 



PART II. SYSTEMATIC HISTOCHEMISTRY 



VI. Inorganic Substances 29 



A. Metallic Elements 29 



B. Nonmetallic Elements 46 



VII. Organic Substances 50 



A. Saccharides 50 



General Principles of the Histochemical Demonstration 



of Saccharides 52 



I. Substances Other than Nucleic Acids .... 61 



II. Nucleic Acids 77 



Appendix: Ascorbic Acid 90 



B. Lipids 91 



Histochemical Methods for Lipids 95 



Physical Methods 95 



Chemical Methods 99 



C. Proteins, Amino Acids, and Products of Protein Me- 



tabolism Ill 



Proteins HI 



Amino Acid Components of Proteins 112 



Antigens 116 



Urea 117 



Uric Acid US 



D. Prosthetic Groups 120 



Phenolic Substances Especially Polyphenols . . . 120 



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