158 Microscopic Histochemistry 



tions; if desired, counterstain with lithium carmine. Mount 

 in glycerin-jelly. The preparations are not permanent. A posi- 

 tive reaction consists of a blue color. 



2 ) For fat peroxides. ( a ) Stable or M nadi reaction.— Fix 

 in formalin-alcohol or formalin. Frozen sections are prefer- 

 able, but excellent results are often obtained with paraffin 

 sections. Smears should be fixed in 95 per cent alcohol or 

 formalin-alcohol (1:9). The incubating mixture is similar to 

 the previous one, except for the pH. Instead of phosphate 

 buffer, use distilled water, and add 2-3 drops of strong am- 

 monia water or N NaOH to 50 ml. of the mixture. A positive 

 reaction consists of a blue color. 



The results of this method can be checked by the {h) 

 Glavind-Granados-Hartmann-Dam technique.'^"' Prepare two 

 solutions: A, dissolve 40 mg. hematin in 10 ml. of pyridine, 

 add 20 ml. of glacial acetic acid; B, dissolve 25 mg. of leuco- 

 2,6-dichlorophenolindophenol in 3.5 ml. of absolute alcohol, 

 add 5 ml. distilled water. This latter solution is unstable and 

 must be used fresh. Mix 0.75 ml. of solution A with the total 

 of solution B and apply the mixture to the sections (or 

 smears ) for 3-5 minutes. Wash in water, mount in glycerol- 

 jelly. Fatty peroxides stain red. 



B) Nonspecific phenol oxidase.— 



Dark brownish or purplish staining of the granules of mye- 

 loid leucocytes can be obtained by incubating alcohol- or 

 formalin-alcohol-fixed smears with slightly alkaline solutions 

 of various phenols, such as tyrosin, adrenalin, hydroqui- 

 none,^^ alpha-naphthol,^^"^^ and dioxyphenylalanin.^^ The 

 conditions of this reaction have not been investigated in de- 



47. Glavind, I., Granados, H., Hartmann, S., and Dam, H.: Experientia, 

 5:84, 1949. 



48. Kreibich, C: V\^ien. klin. Wchnschr., 23:701, 1910; Kreibich, C: 

 ibid., 23:1443, 1910. 



49. Loele, W.: Miinchen. med. Wclmschr., 57:1394, 1910. 



50. Loele, W.: Die Phenolreaktion ( Aldaminreaktion ) und ihre Bedeu- 

 tung fiir die Biologic (Leipzig: W. Klinkhardt, 1920). 



51. Loele, W.: Virchows Arch. f. path. Anat., 262:39, 1926. 



52. Bloch, B., and Peck, S. M.: Folia haemat., 41:166, 1930. 



