268 



Microscopic Histochemistry 



Di-isopropyl fluorophosphate, 210, 



211 

 p-Dimethylaminobenzaldehyde, re- 

 agent for amino acids, 115 

 p - Dimethylaminobenzylidenerhoda - 



nine, reagent for copper, 41 

 Dinitroresorcinol, reagent for iron, 40 

 Diphenylthiocarbazide 

 reagent for mercury, 44 

 reagent for zinc, 42 

 Diphenylthiocarbazone, reagent for 



mercury, 44 

 Dipicrylamine, reagent for potassium, 



31 

 Disulfides, 128 

 Ditetrazolium chloride, reagent for 



dehydrogenases, 152 

 Dithiooxamide, reagent for copper, 



41 

 Dithizone, reagent for mercury, 44 

 Dopa oxidase, 159 



method, 160 

 Double azo-coupling, 77 

 Dyes, oil-soluble, 96 



Elastic fibers, SchiflF reaction of, 66 



Elements 

 metallic, 29 

 nonmetallic, 46 



Embedding, methods of, 15, 16 



EnterochromaflBn cells, fluorescence 

 of, 125 



Enterochromafiin substance, 120, 124 

 azo-coupling reaction, 124, 127 

 indophenol reaction, 124, 128 



Enzymatic methods, sources of error 

 in, 142-150 



Enzymatic reactions, control of, 149 



Enzymes, 137 

 fixation for, 138 

 general technique for, 138 

 histochemical quantitation, 23 



histochemical reactions for, 141 

 hydrolytic, 167 

 oxidative, 150 

 specificity of, 149 



Esterase 



indoxyl acetate substrate for, 208 

 o-naphthol method for, 206 

 /3-naphthol method for, 208 

 naphthol AS method for, 207 



Tween method for, 203 

 a-Esterase, 212, 215 



Esterases 



azo dye methods for, 206 

 biochemical classification, 201 

 histochemical classification, 215 

 difi^erences between, 212-15 



Estrone, 109 



Extraction procedures for nucleic 

 acids, 86 



False localization of enzymatic re- 

 actions, 144 

 False negative reactions in enzy- 

 matic histochemistry, 143, 183 

 False positive reactions in enzymatic 



histochemistry, 148, 183 

 Fatty acids, 92, 99 

 metachromasia of, 99 

 unsaturated, 101 



auto-oxidation of, 101, 105 



Schiff reaction of, 109, 110 



Fatty peroxides, 101, 110, 156, 157 



methods, 158 

 Fatty substances, classification, 92 

 Ferric chloride, reagent for phenolic 



substances, 121 

 Ferriferricyanide, reagent for sulf- 



hydryl groups, 129 

 Ferrocyanide 



reagent for copper, 41 

 reagent for iron, 37 

 reagent for proteins, 112 

 Feulgen's nucleal reaction 

 method, 83 



of nervous elements, 82 

 validity, 80 

 Fibrin, 63 

 Fixation 



of enzymes, 138 

 of glycogen, 61 

 of lipids, 95 

 methods of, 14 

 Flavoproteins, 130 

 Fluorescein isocyanate, couphng of 



proteins with, 116 

 Fluorescence, 8 



of enterochromaffin cells, 8, 125 

 of flavoproteins, 130 

 of Hpids, 98 



