220 Microscopic Histochemistry 



0.2 M MALE ATE BUFFER 



50 ml. 0.4 M acid Na maleate (2.32 g. of maleic acid + 20 ml. of 

 N [4 per cent] NaOH in 50 ml.) + x ml. of 4 per cent NaOH diluted 

 to a total of 100 ml. 



Add a few crystals of camphor. 



0.2 M PHOSPHATE BUFFER 



Solution A: monobasic Na phosphate (NaH2P04 • HgO), 2.76 g. in 

 100 ml. 



Solution B: dibasic Na phosphate (Na2HP04 • 7H2O), 5.36 g., or 

 Na2HP04 • I2H2O, 7.6 g. in 100 ml. 



0.2 M tris(hydroxymethyl)aminomethane-maleate- 



(tris-maleate) buffer 



Dissolve 29 g. of maleic acid and 30.3 g. of tris(hydroxymethyl)- 

 aminomethane (Commercial Solvents Corp., New York 17) in 500 

 ml. of distilled water. Add about 2 g. of charcoal, shake, let stand for 

 10 minutes, and filter. 40 ml. of this stock solution + x ml. of N (4 

 per cent) NaOH diluted to a total of 100 ml. 



Add a few crystals of camphor. 



