DISCUSSION 



1" Mitchell, J. S. and Simon-Reuss, I. Brit. J. Cancer, 1952, 6 317. 

 " Hughes, A. and Simon-Reuss, I. ibid, 1953, 7 142. 



12 Friedmann, E., Marrian, D. H. and Simon-Reuss, I. Brit. J. Pharmacol. 1948, 



3 263. 



13 Friedmann, E., Marrian, D. H. and Simon-Reuss, J. ibid, 1948,3 335. 

 1^ Friedmann, E., Marrian, D. H. and Simon-Reuss, J. ibid, 1949,4 105. 

 15 Marrian, D. H. J. Chem. Soc. 1949, 1515. 



i« Marrian, D. H. ibid, 1949, 1797. 



17 Friedmann, E. and Bailey, N. T. J. Biochim. Biophys. Acta, 1950, 6 274. 



IS Friedm.\nn, E., Marrian, D. H. and Simon-Reuss, I. ibid, 1952, 9 61. 



19 Friedmann, E., Marrian, D. H. and Simon-Reuss, I. ibid, 1952, 8 504. 



20 Friedmann, E. ibid, 1952, 9 65. 



21 Friedmann, E. Brit. J. Radiol. 1954, 27 137. 



22 Gellhorn, a. and Gagll\no, T. Brit. J. Cancer, 1950, 4 103. 



23 BERKM.A.N, A. Tiirkiye Tip Enciimeni Arsive {Istanbul), 1951, 2 1. 

 Berkman, a. Seventh Int. Congress Radiology Abstract TWO, 1953. 



2* JoLLES, B. Ann. Rep. Brit. Empire Cancer Campaign, 1952, 30 324. 



25 DiTTRiCH, W. and Schmermund, H.-J., Strahlentherapic, 1953, 90 88. 



2 6 Cater, D. B. and Phillips, A. F. Nature, Land. 1954, 174 121. 



2' Domagk, G., Peterson, S. and Gauss, W. Z^- Fd. Krebsforsch. 1954, 59 617. 



28 Fieser, L. F., et al. J. Amer. Chem. Soc. 1948, 70 3215. 



29 Ball, E. G., Anfinsen, C. B. and Cooper, O. J. biol. Chem. 1947, 168 257. 



30 Potter, V. R. and Reif, A. E. ibid, 1952, 194 287. 



Fisher, R. A. Statistical Methods for Research Workers, Oliver and Boyd, Ltd.. 



Edinburgh, 1925-46. 



Fisher, R. A. The Designof Experiments, Oliver and Boyd, Ltd., Edinburgh, 1935-46. 



Medical Research Council. Brit. Med. J. 1948, 2 769. 



Medical Research Council, ibid, 1950, 2 1073. 



Hill, A., Bradford, Principles of Medical Statistics, Pub. by Lancet, Ltd., London, 



1952, Fifth Edn., pp. 5, 233. 



31 KLA.RNOFSKY, D. A., BURCHENAL, J. H., ArMISTEAD, G. C, Jnr., SOUTHAM, C. M., 



Bernstein, J. L., Graver, L. F. and Rhoads, C. P. Arch. Int. Med. 1951, 87 477. 



DISCUSSION 



S. Neukomm : For four years, our research team has devoted part of its activity 

 to the study of the metabolism of Synkavit, injected in rats, and its mode of action 

 in vitro on fibroblast from the heart of mice. We used Synkavit labelled with radio- 

 active phosphorus*. This substance undergoes a more or less rapid hydrolysis in 

 the organism, according to the nature of the organs in which it is fixed. This 

 hydrolysis brings about the liberation of the phosphoric groups (PO4), which then 

 follow the general metabolism of the PO4 ions. From then on, the destiny of the 

 basic molecule (2-methyl-l, 4-naphto-hydroquinone) can no longer be followed. 

 Still, it is most probable that this molecule remains locally fixed in the cells in an 

 oxidized form, as Mitchell's report seems to show. 



With reference to the distribution of Synkavit in the various organs of rats, our 

 results confirm and extend Mitchell's statements. Synkavit is mainly fixed and 

 accumulates (7) in the organs which have intense cellular proliferation centres and 



* Neukomm, S., Peguiron, L., Lerch, P., and Richard, M. Arch, internal. Pharmacodyn 

 Therapie, XCIII, 1953, 373. 



Peguiron, L. and Neukomm, S. Acta Anat. 1954, 21 46. 



189 



