NEUTRON EFFECTS ON ANIMALS IQl 



listed in Table VI, show that sonic treatment caused no significant change 

 in the proteins of plasmas C and E diluted with three parts of buffer 

 solution, nor of plasma B, whether diluted or undiluted. Defibrination 

 occurred during dialysis of the sonicized sample of plasma D: but since 

 defibrination has occasionally been observed during dialysis of plasmas 

 which have not been sonicized, it is believed that the defibrination was 

 caused by the undetermined factor operating in these other instances, rather 

 than by the sonic treatment. 



Electrophoretic Analysis of Bone Marrow Proteins. The bone marrow 

 extracts from the three non-irradiated rabbits, B, D, and E, gave the elec- 

 trophoresis diagrams shown at the middle of Fig. 2. By comparing these 

 with the plasma diagram at the top of the figure, it can be seen that the 

 bone marrow constituents had approximately the same mobilities as the 

 plasma proteins, but were present in different proportions than in plasma. 

 The composition of the three extracts was quite uniform (Table VII): 

 each contained about 25 per cent of albumin, 15 per cent of a and /3 globulin, 

 55 per cent of fibrinogen, and 10 per cent of y globulin. In view of the 

 high albumin content of rabbit plasma, the comparatively low albumin 

 content of the bone marrow extracts is an indication that the bone marrow 

 proteins were, at most, only slightly contaminated by plasma proteins 

 from residual blood in the marrow. 



A second analysis was made on bone marrow extract B after two months 

 in the refrigerator; the remaining solution was reduced to half its original 

 volume by pressure dialysis. The resulting electrophoresis diagi-am is given 

 by the dotted curve for extract B in Fig. 2. The good agreement shown 

 by the two experiments (see also Table VII) is evidence for the stability of 

 the extract. 



The bone marrow extracts of the two irradiated rabbits, A and C, were 

 turbid. Part of each was clarified by centrifuging for an hour at about 

 10,000 r.p.m. in a multispeed attachment with dry ice cooling. Electro- 

 phoresis was carried out on both the clear and the turbid portions of each. 



The clarified portions of the extracts gave the electrophoresis diagrams 

 reproduced at the bottom of Fig. 2. For rabbit A, obtained one day after 

 irradiation, there was no significant difference from the non-irradiated 

 rabbits. For rabbit C, sacrificed 12 days after irradiation, the percentages 

 of albumin, a globulin and /3 globulin were higher, and the percentages of 

 fibrinogen and y globulin were lower (Table \ll). The turbid portion of 

 extract C showed the same difference with respect to the extracts from the 

 non-irradiated rabbits. This is demonstrated in Fig. 3, in which the 

 dashed lines represent the diagram for rabbit C, and the solid line the 

 diagram for rabbit D, which was not irradiated. The dotted line in this 

 figure represents extract C clarified. Centrifugation resulted in a reduction 



