98 



NEUTRON EFFECTS ON A.NIMALS 



intestine were necrotic although the epithelium of the mucous membrane 

 was normal. The spleen showed marked atrophy of the malpighian cor- 

 puscles with abundant blood pigment in the monocytes. In general, a 

 diagnosis of necrosis and atrophy of lymphoid tissue was made. However, 

 it is difficult to ascribe these findings to the irradiation given the previous 

 day. Although atrophy of lymphoid tissue normally follows neutron 

 irradiation, it is not so pronounced one day after irradiation. Apparently, 



ASCENDING 



«- 



DESCENDING 

 ► 



Fig. 3. Tracings of Longsworth diagrams obtained by electrophoresis of rabbit 

 bone marrow extracts for 30 minutes at 27 ma (48.6 coulombs) in 0.1 X sodium veronal- 

 veronal buffer solution of pH 8.6. Solid line : bone marrow extract D, no irradiation. 

 Dashed line: bone marrow extract C, 12 days after irradiation with 135 n. Dotted 

 line: bone marrow extract C, clarified by centrifuging. 



this animal was ill prior to irradiation although no indications thereof were 

 given by any of the data obtained prior to irradiation. 



ELECTROPHORESIS EXPERIMENTS 



All electrophoresis experiments were carried out in the Tiselius apparatus 

 with the single-section cell of 11 ml. capacity, using a 0.1 X sodium veronal- 

 veronal buffer solution of pH 8.6. For the plasma and serum solutions 

 the current was kept at 20 ma, and for the bone marrow extracts at 27 ma. 

 Photographs were taken by both the Longsworth (7) and Svensson (8) 



