NEUTRON EFFECTS ON ANIMALS 



91 



the serum and plasma were diluted, immediately after removal of the 

 cellular constituents, with three parts of buffer solution, then dialyzed 

 against two changes of the same buffer. The nitrogen detenninations are 

 summarized in Table II and the electrophoretic data in Tables III, IV, V, 

 and YI. The electrophoretic diagrams for a representative plasma and 

 serum are given at the top of Fig. 2. 



DESCENDING . 



Fig. 2. Tracings of Svensson diagrams obtained by the electrophoresis of rabbit 

 plasma, rabbit serum, and rabbit bone marrow extracts in 0.1 X sodium veronal- 

 veronal buffer solution of pH 8.6. Plasma D-1 : 95 minutes at 20 ma (114 coulombs) ; 

 serum D-1 (dotted curve): 92 minutes at 20 ma (110.4 coulombs). Bone marrow 

 extracts: 70 minutes at 27 ma (113.4 coulombs). Plasma and serum D-1 and bone 

 marrow extracts D, E, and B: no irradiation. Bone marrow extract A: 1 day after 

 irradiation with 135 n; solution clarified by centrifuging. Bone marrow extract C: 

 12 days after irradiation with 135 n; solution clarified by centrifuging. Bone mar- 

 row extract B, dotted curve: solution concentrated to half the volume two months 

 later. 



Bone Marrow. To obtain the bone marrow for study, the rabbits were 

 exsanguinated from the heart, plasma and serum being prepared from this 

 blood for both chemical and electrophoretic analysis. Immediately after 

 death and as rapidly as possible, 10 long bones (2 each — femur, tibia, 

 humerus, ulna and radius) were removed and freed of all adherent tissues. 

 The cleaned bones were then frozen in solid carbon dioxide — a process that 



