IRRADIAl 1().\ AM) RI.IM.ACEMENI' OK MOM, MARROW, USING i»«AU 



The work was supported by a grant Jruiii The Australian Atomic Energy Commission 

 and one of us {F. R.) would like to acknowledge a maintenance grant also from the 

 Commission. 



We would like to thank the Boards of St. VincenCs Hospital and the Royal Alexandra 

 Hospital for Children, particularly Dr. D. Reye, for providing facilities for carrying 

 out the investigation. 



We are grateful to the Commonwealth X-Ray and Radium Laboratory for obtaining 

 all the radio-active materials used in the investigation. 



REFERENCES 



^Barnes. D. \V. H., Corp, M. J., Lounr, J. F. and Neal, F. E. Brit. mcd. J. ii 



(1956) 626 

 2 Porter, K. A. Brit. J. exp. Path. 38 (1957) 401 

 ^Osgood, E. ¥.. Therapeutic Use of Artificial Radio-isotopes (Ed. P. F. Hahn): John 



Wiley and Sons, New York, 1956 

 *Sheppard, C. W., Wells, E. B., Hahn, P. F.. and Goodell, J. P. B. J. Lab. din. 



A/f^. 32 (1947) 274 

 ^DoBsoN, E. L., GoFMAN, J. W., JoNEs, H. B., Kelly, L. S. and Walker, L. A. 



/. Lab. din. Med. 34 (1949) 305 



sMayneord, W. V. Brit. J. Radiol. N.S. Supplement 2 (1950) 142 

 ^RooT, S. W^., Andrews, G. A., Kniseley, R. M. and Tyor, M. P. Cancer, WY. 



1 (1954) 856 

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Therapeutic Use of Artificial Radio-isotopes (Ed. P. Y. Hahn): John Wiley and Sons, 



New York, 1956 

 "Jacobson, L. O., Marks, E. K. and Gaston, E. O. Rev. Hemat. 8 (1953) 515 

 ^iJacobson, L. O., Robson, M.J. and Marks, E. K. Proc. Soc. exp. Biol., A'.V. 75 



(1950) 145 

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DISCUSSION 



Mr. van den Brenk: Could I ask how long after the last graft was given, the marker 

 was identified, and also could you give us an idea of the relative dosage with regard 

 to the biotin of the animals and whether they were grafted or not grafted ? How 

 long did they live after irradiation, and what was the dosage given ? 



Dr. George : First of all, as to the time of identification of the marker — that was 

 three weeks following bone transplantation. The figures you are asking for (con- 

 cerning the survival or otherwise of the rabbits that had different dosages), are all 

 in the table. 



Dr. Hoffman: Two points I should like to raise Dr. George — firstly, do you have 

 any evidence as to whether, although the spleen has taken up so little isotope, it in 

 fact, offers any protective effect? 



Dr. George: Only indirectly, in that we were puzzled by the fact that animals that 

 had been receiving 6,000 r to the marrow (and we took slides of the marrow and it 

 really was fairly aplastic), still recovered, and only indirectly did we infer that spleen 

 protection was operating. We just have not had time to do everything, but we 

 noticed that the spleens were given very low dosages and therefore, we cut a corner 

 by not proving the point, but in future, splenectomizing all rabbits. 



66 



