Oxidative Phosphorylation in Irradiated Cells 89 



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DISCUSSION 



Loutit: It is very heartening to me to see this correlation between 

 biochemical and histological findings, for so rarely do we have the two 

 together. I would like to ask whether the cytologist or histologist was 

 always doing his work unseen as it were; did he know what he was 

 looking at, whether it was a half-hour, two-hour or four-hour section; 

 or could he have been biased by a previous knowledge of the time at 

 which the section was taken? 



Van Bekkum: The histologist, Dr. O. Vos, only knew that some of the 

 sections were taken at say 30 minutes and others 1,2 or 4 hours after 

 irradiation, but he did not know which one. On the basis of nuclear 

 changes he could not differentiate between 15 minutes after irradiation, 

 30 minutes after irradiation and control sections, but he could differen- 

 tiate between them because of the decrease of mitotic figures in the 

 slices from irradiated rats. 



Roller: I have seen lymphocytes, irradiated with quite small doses, 

 which are undergoing nuclear disintegration similar to that described 

 by Dr. Van Bekkum. The degeneration of the lymphocytes in extremely 

 high numbers cannot be explained by the old theory that the cells have 

 gone through mitosis after irradiation and die because of chromosome 

 fragmentation. That raises a very important point which is included 



