160 Discussion 



in 28 per cent protein solution as described by Baner, Joseph and 

 Esnouf (1955, Science, 123, 24). 



Stapleton: Someone in Jacobson's group did a very nice experiment 

 using leukaemia cells, making a nuclear fraction, and from the nuclear 

 fraction they were able to transplant leukaemia to another animal. I 

 think they found also that the leukaemia cells were acted upon by the 

 same enzymes that Cole proposed were not effective on whole cells. 

 This seems to me to be good proof that there were cells in the nuclear 

 fraction, plus the fact that the enzymes did work on some whole cells. 



Stocken: Yes, Jacobson came to the conclusion that he needed 100,000 

 cells. 



