Nucleic Acids and Amino Acid Incorporation 181 



cent inhibition whereas 10"^ m is markedly less inhibitory. 

 Fig. 1 shows the effect of oxine on glycine incorporation in 

 nucleic acid-depleted cells incubated in the presence and 

 absence of staphylococcal DNA; 10 ~^ M-oxine prevents the 



ISOOt 



1600- 



X 



o 



1400 



2 1200 



CO 



o 

 o 



z 

 o 



1000 



800- 



< 

 a: 



2 600 ^ 



O 

 o 



Z 



400 



o 



^ 200 

 o 



oi 



+ DNA 



O CONTROL 



r 

 6 



5 4 



LOG MOLAR C0NC.8-KYDR0XYQUIN0LINE 



-I 

 3 



Fig. 1. Effect of 8-hydroxyquinoline on the incorporation of glycine 

 in disrupted staphylococcal cells depleted of nucleic acid and in- 

 cubated in the presence and absence of added staphylococcal 



deoxyribonucleic acid. 



stimulation of incorporation by DNA. The effect is not specific 

 for DNA since stimulation by RNA or by purified preparations 

 of the "glycine incorporation factor" is abolished in the same 

 way and at the same concentration. Albert, Gibson and 

 Rubbo (1953) found that the bactericidal action of oxine 

 depends upon the presence of heavy metal ions in the medium 

 and that toxicity was maximal when equimolar quantities of 



