lAA OXIDASE INHIBITOR AND MORPHOGENESIS 151 



State for long periods. Some quantitative data on recovery after adsorp- 

 tion and elution are presented in Table V. 



Table \'. Recovery of Inhibitor Activity after Adsorption on Charcoal and 



Ekition with Various Substances 



Chromatographic purification of the eluted inhibitor was next at- 

 tempted on a variety of materials. The best results were obtained with 

 columns of cellulose powder and on paper strips. Some representative 

 procedures and results are presented in the paragraphs below. 



A. Chromatography on a cellulose column (Whatman cellulose pow- 

 der) 

 Column size: 27 cm high X 1 cm wide. 

 Sample: Charcoal-treated preparation. 

 Flow rate: 1 ml/lOmin. 

 Fraction size: 1.7 ml. 

 Elution results 



1. Solvent: n-butanol; 100 ml collected. Fractions 9-13 (break- 

 through) contained UV absorbing material; fractions 14-60 

 contained none. 



2. Solvent: ^7-butanol (86) : water ( 14) ; 240 ml collected. Ultra- 

 violet absorption spectrum records indicated definite fraction- 

 ation of components of the mixture. 



3. Column extruded, cellulose washed with water. Ultraviolet 

 absorption of this fraction was appreciable. 



Fractions were combined as seemed best according to the spectra. The 



