152 PHOTOCONTROL OF GROWTH 



solvents were removed in vacuo, 40°, and the residues dissolved in 



small amounts of water. 

 Enzymatic assay results 



Fractions collected under all three elution conditions which had 

 appreciable UV absorption also showed inhibitor activity. Re- 

 covery was approximately 90% of the original. 

 Paper strip chromatography of eluates from above procedure 

 Paper: Whatman #1. 



Solvent system: «-butanol (4): acetic acid (1): water (5) (or- 

 ganic phase). 



1. Several UV-absorbing components were observed from each 

 spot. Those from different fractions gave different Rf values. 



2. Inhibitor activity was diffuse and not found in the spots show- 

 ing greatest UV absorption. It could be recovered only when 

 large areas of the chromatogram were recombined. In some 

 cases activity at a well-defined Rf could be shown. 



Conclusions 



1. This method offers an opportunity for fractionating the solu- 

 tion containing the inhibitor. 



2. The results suggest that not one but several compounds with 

 inhibitor activity are present in the original extract. 



B. Paper strip chromatograms 



The best solvent systems used were: 



1. /7-butanol: H-O. 



2. /7-butanol (4): acetic acid (1): water (5) (organic layer). 



3. z-amyl alcohol (4): acetic acid (1): water (5) (organic 

 layer). 



4. r-butanol (7): NH3 (1): H2O (2). 



5. 6% acetic acid. 



Other systems tested include the following in which there was no 

 movement: 



1. «-butanol (9): NH. (1). 



2. benzene (6): acetic acid (7): HjO (3). 



3. benzyl alcohol (4): acetic acid (1): H2O (10). 



Paper chromatography run under rigidly standardized conditions 

 thus appears to represent a good potential tool for the separation of the 



