?to 



UADI A'l'ION Hl(H.(KiY 



0.200 



4 00 



600 



800 



0.600 



800 



100 

 EXTINCTION 



Fig. 6-14. Typical distrilnition curves of microspectrophotometric data. Curve I, 

 sections of nucleoli, corn pollen mother cells, Millon reaction ; curve II, nuclei of mouse 

 sarcoma, methyl green; curve III, nucleoli, corn pollen mother cells, ultraviolet 

 absorption; curve IV, mouse spermatocyte nuclcM, Feulgen reaction; curve V, pre- 

 leptotene nuclei from mouse testis, Feulgen reaction. {After Pollister and Swift, 1950.) 



Table 6-5. Liver Nuclei, Ultraviolet Absorption 



The reproducibility of ultraviolet absorption of 5.0-m sections of nuclei of the liver 

 of the salamander, Amhh/stoma, is shown by data from different slides of the same 

 organ, measured at different times. Fixation in acetic acid-alcohol, eml^edded in 

 paraffin, mounted in glycerin, measured as absorption of 254 ni/u radiation isolated 

 from a Hanovia SC2537 mercury-vapor lamp by a Zeiss two-prism quartz mono- 

 chromatic illuminator, with glycerin immersion monochromatic objective, N.A. 0.85, 

 condenser aperture about N.A. 0.40. 



