100 THE ACTION OF THE LIVING CELL 



was ligated and a small quantity of the chemical to 

 be studied was injected below the site of the ligature. 

 By such means, free diffusion of the reagent was pre- 

 vented and the tissues adjacent to the site of the in- 

 jection received the brunt of the action. By this means, 

 a greater tissue damage and consequent increase in 

 the amount of cytost liberated was achieved, for the 

 oxidation or elimination of the injected substance was 

 prevented. Upon removal of the ligature, the accu- 

 mulated cytost was released into the circulation and 

 carried to the viscera, which reacted in the manner 

 previously described. 



Cat No. XIX. 



March 1, 1919. Leg tied off. 3 cc. alcohol injected below ligature. 



Two hours later ligature released. Leg swollen. 

 March 7. Swelling of leg has disappeared, animal becoming thin. 

 March 10. Animal failing rapidly. Skin excoriating over site of 



injection. 

 March 11. Found dead. 



Autopsy: Lungs, partially consolidated ; stomach, duodenum, 

 and liver, markedly congested; brain, meninges congested; 

 heart, ventricles normal. 



Cat No. XX 



March 1, 1919. Leg tied and 4 cc. ether injected below ligature. 



Latter removed after 2 hours. 

 March 4. Leg swollen, animal quiet and stupid. 

 March 10. Does not eat, thin, weak. 

 March 13. Found dead, body still warm. 



Autopsy: very thin, fat and muscles gone. Lungs, congested, 

 hemorrhagic, red, solid ; stomach and duodenum, congested ; 

 liver, congested, fatty degeneration; kidneys, congested, fatty; 

 spleen enlarged; brain, normal. 



