236 THE ACTION OF THE LIVING CELL 



cause death within fifteen to twenty minutes was al- 

 ways greater than in the case of guinea pig tissue ash. 



Such differences in the toxicity of the tissue ash of 

 various species was also observed when they were in- 

 jected intravenously, the route which causes the most 

 rapid onset of shock. For comparative purposes 10% 

 solutions, in normal saline, of the ashes of various ani- 

 mal tissues were employed and were injected into the 

 jugular veins of the experimental animals. 



When injected in this fashion into rats weighing 

 150-200 gms., a 10% solution of the ash of rat tissues 

 was found to cause the onset of shock and death in from 

 5 seconds to 2 minutes. When rats of the same size 

 received }i cc. of a 10% solution of cat tissue ash, 

 they developed a slight shock, but usually recovered 

 in about 10 minutes. This result shows definitely the 

 existence of a species specificity in the ash. 



A large number of similar experiments were carried 

 out with cats, using 10% solutions of the ash obtained 

 from rat, horse, beef, and lion tissues. When 1 cc. of 

 such a solution of homologous tissue ash was injected 

 into cats weighing from 2500 to 3000 gms., it was 

 found that the animals always passed into severe shock 

 and frequently died immediately. When such was not 

 the case, the cats always died within two or three days 

 after the intravenous injection of this quantity of cat 

 tissue ash. When like quantities of the tissue ash of 

 horse, dog, beef, or lion muscle were substituted for 

 the cat ash solution, the injected cats of similar weight 

 often showed no ill effects whatsoever. At other times 

 they developed a mild shock from which they made 

 uneventful recovery within less than an hour. 



For some curious reason the ash obtained from rat 



