APPENDIX 291 



making 10.000 r.p.m., for Yi minute. Animal ap- 

 peared depressed with slight symptoms of shock. Ob- 

 served for 10 minutes and then placed in proximity 

 to centrifuge arm for another half minute. Well de- 

 veloped shock, eyes dull, fur staring. 

 845 AM. Temperature 35°. Marked trembling. 

 1.05 PM. Temperature 36°. 



3.45 P.M. Animal in crazed condition, began to eat 

 feces and chewed frantically at steel forceps under 

 jar. 



6.00 P.M. Autopsied. Upper part of intestines and 

 stomach markedly congested and hemorrhagic. Liver 

 showed extensive small hemorrhagic areas. Kidney 

 and spleen hemorrhagic. Brain and spinal cord find- 

 ings were practically negative although the mem- 

 branes were congested. 



A guinea pig, weight 775 gms., placed in proximity 

 to centrifuge arm as above described for two ^ min- 

 ute periods spaced 5 minutes apart. Slight symptoms 

 of shock from which the animal recovered. Although 

 kept upon a normal diet this animal lost weight. 10 

 days after the experiment it weighed 649 grams (loss 

 126 grams). Was dull and listless with dull, rough 

 coat. 



This experiment offers evidence of cytost intoxica- 

 tion following an injury of a sort which leaves no 

 definite external indication of its nature. 



EXPERIMENTS WITH AUTOLYZED TISSUE 



For experiments such as those described below, 

 sterile autolyzed tissue extracts are easily prepared 

 as follows : The desired tissue is removed aseptically 



