APPENDIX 293 



thetic, the animal regained consciousness promptly, 

 but severe shock soon supervened. The injection was 

 made at 4.30 P.M. when the rectal temperature was 

 39.4°. Following the injection the temperature 

 dropped regularly, reaching 34.1 ° at 8.45 P.M., when 

 the animal died in shock. 



Rabbit, weight 1,200 gms. 1:30 P.M. Rectal tem- 

 perature 39°. Injected 2.5 cc. of the autolysate into the 

 marginal vein of the ear. Symptoms of shock began 

 at 2.00 P.M., the animal appearing apathetic. 2.30. 

 Eyes dull, gums pale. 3.30. Reflexes diminished, 

 marked pallor of mucous membranes of mouth, nose, 

 and conjunctivae, pulse, rapid and weak, labored 

 breathing, rectal temperature 37°. Temperature 34° 

 2.1 8. 45P.M. T)'itd9.00. Immediate autopsy disclosed 

 marked congestion of stomach, duodenum, upper 

 small intestines, and liver. 



That extracts of charred tissues obtained from 

 burns behave similarly to tissue autolysates is shown 

 by the following experiments: 



One half gram of charred tissue was dissected from 

 the burned area on the back of a cat which had passed 

 into shock as a result of a burn. This was ground in 

 a mortar with 5 cc. of saline and filtered. 2 cc. of this 

 extract were then injected into the jugular vein of a 

 cat weighing 2,200 gms. Shock developed immedi- 

 ately following the injection, but the animal recovered 

 after several hours. 



One cc. of this extract was injected into the mesen- 

 teric vein of a cat weighing 2,500 grams. Death fol- 

 lowed within 3 minutes. 



Ten grams of rabbit muscle were charred in a 

 crucible over a Bunsen flame. The black mass was 



