96 



LIGHT AND LIFE 



Polarization Spectra of Tyrosine, 

 Ribonuclease, Insulin, and Zein 

 in Propylene Glycol at -70°C 



22 



20- 



■18 

 ■I 



14 

 ■12 



■08 

 ■06 

 04 

 02 

 O 

 02 

 ■04 

 06- 

 08 



Tyrosine 

 Insulin 

 Zein 

 Ribonuciease 00 



Tyrosine 



■o- o Ribonuciease 

 Zein 



-«--x Insulin 



230 240 250 260 , 270 280 290 300 



\ 



Fig. 10. Polari/ation spcdra of tyrosine, ribonuciease, insulin, and zein in organic 



solvent at — 70°C. 



Excitation and Fluorescence Spectra 

 of Proteins 



18 — 

 17 — 



16 



i 14 



C 13 



I 12 

 "- 11 



10 



1 9 

 ?■ 8 

 S 7 

 < 6 

 « 5 



4 



o 



V 



FLUORESCENCE 

 EXCITATION 

 BANDS 



FLUORESCENCE 

 BANDS 



^Chymotryps 



250 



300 350 



Wavelcnqth A {«iYl 



400 



I-ig. 1 1. Muorescence excitation and emission spectra of pepsin and chymotrypsin. 



