n . 1). McELliOY AND H. H. SELIGER 



245 



100 



V) 



z 



liJ 



1- 

 z 



> 



I- 

 < 



700 



Fig 24. Fluorescence spectra of chemicallv svntliesi/cd LAMP and LHo-AMP in 

 acid solution. The acid condition was retjuircd. since both compounds hvcholvzc 

 rapidly in neutral and basic solutions, giving tiic Huorcscence of free L and LHo 

 respecti\ely. The position of the peak fluorescence f)f LHo-.VMP appears to be a 

 function of pH, and in more acidic solutions there is a red fluorescence. 



ber of photons emitted in a bioliiminescent reaction. The total 

 light emitted during the enzymatic oxidation of luciferin over the 

 range 10-^- mole to 10-^" niole was measured, and from these data 

 we conclude that one light quantum is emitted per luciferin molecule 

 oxidized (24, 23) . 



PH7I ^i. 



500 



550 



600 



ITIfJL 



650 



700 



Fig. 2."). Fmission spectra of firefly bioluminescencc for various f)U values in 

 glycylglycine Inilfer solution. These spectra were measured with an RC.\ 7320 

 phototube sensitive out to 8000 A. 



