m) 



LIGHT AND LIFE 



Compound 

 Sodium formate 

 Sodium citrate 

 Potassium citrate 

 Potassium oxalate 

 Potassium sodium tartrate 

 Sodium succinate 

 Potassium hydrogen phthalate 



TABLE 2 — continued 



Final Molar 

 Concentration Relative Intensity 



bovine serum albuiiiin has a marked stimulatory effect upon both 

 tlie intensity of himinescence and the total light obtained (Fig. 5) . 

 With high concentrations of albumin, the light intensity (rate) is 

 decreased, whereas the total light (yield) from the reaction remains 

 maximal. This effect of albiunin has been shown to be due to the 

 formation of an albumin-luciferin complex which does not so readily 

 undergo nonenzymatic dark oxidation. 



The effect of concentration of several salts upon the light intensity 

 of the reaction is shown in Fig. G. Remarkably high concentrations 

 are required for maxinunn activity. Most of the curves do not inter- 

 sect the origin, since buffer was present (see Fig. 1). VV'ith no resi- 



